Complete genome sequence of the Medicago microsymbiont Ensifer (Sinorhizobium) medicae strain WSM419

Wayne Gerald Reeve, Patrick Chain, Graham O'Hara, Julie Ardley, Kemanthi Nandesena, Lambert Bräu, Ravi Tiwari, Stephanie Malfatti, Hajnalka Kiss, Alla Lapidus, Alex Copeland, Matt Nolan, Miriam Land, Loren Hauser, Yun-Juan Chang, Natalia Ivanova, Konstantinos Mavromatis, Victor Markowitz, Nikos C. Kyrpides, Margaret Gollagher, Ron Yates, Michael Dilworth, John Howieson

Abstract


Ensifer (Sinorhizobium) medicae is an effective nitrogen fixing microsymbiont of a diverse range of annual Medicago (medic) species. Strain WSM419 is an aerobic, motile, non-spore forming, Gram-negative rod isolated from a M. murex root nodule collected in Sardinia, Italy in 1981. WSM419 was manufactured commercially in Australia as an inoculant for annual medics during 1985 to 1993 due to its nitrogen fixation, saprophytic competence and acid tolerance properties. Here we describe the basic features of this organism, together with the complete genome sequence, and annotation. This is the first report of a complete genome sequence for a microsymbiont of the group of annual medic species adapted to acid soils. We reveal that its genome size is 6,817,576 bp encoding 6,518 protein-coding genes and 81 RNA only encoding genes. The genome contains a chromosome of size 3,781,904 bp and 3 plasmids of size 1,570,951, 1,245,408 and 219,313 bp.  The smallest plasmid is a feature unique to this medic microsymbiont.

DOI: 104056/sigs.43526

Keywords


Root nodule bacterium, microsymbiont, Sinorhizobium, nitrogen fixing, legume

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Acknowledgements

We would like to gratefully acknowledge the support of many members of the Genomic Standards Consortium, the broader genomic science community, and those who have indicated their willingness to serve as editors, reviewers and contributors.

Funding for SIGS is provided by a grant from the Office of the Vice President for Research and Graduate Studies at Michigan State University, the Michigan State University Foundation, and the US Department of Energy Biological and Environmental Research DE-FG02-08ER64707.

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